In the present work water quality indices of Mandakini river, Chitrakoot have been determined on the basis of various water quality parameters for 3 seasons, namely post-monsoon and winter at 6 sampling sites. The physico-chemical and biological parameter which were taken into account for the present work are TDS, turbidity, total hardness, chlorides, BOD, DO, total alkalinity, pH and MPN coliforms. Investigations show that all the 6 sampling sites have slight to moderate water pollution and their water can be used for drinking purposes only after proper treatment.