Determination of source of fecal pollution in rural water by the use of antibiotic resistance profiling is promising and the patterns of MAR in different animal populations vary according to the types and quantities of agent used. In the present study, a total of 260 water samples from Akola and Buldhana district were analyzed for water quality and detection of thermotolerant coliform (E.coli), and determined antibiotic resistance analysis from salinity affected villages of Purna river basin of Vidarbha and 243 (95%) water samples were found contaminated by MTFT and 75 (29%) by MFT. The study indicated that the animals were the major source, however man was also a significant contributor of water contamination.