Studies on the population of key pests and natural enemies in RCH 134 Bt and non-Bt cotton hybrid under sprayed and unsprayed conditions were carried out under intercropped conditions. The cotton crop was intercropped with okra, pigeon pea, castor and cowpea to enhance the population build up of cotton jasid, whitefly, spotted bollworm and coccinellids. The pooled data of all the observations recorded significantly lower incidence of spotted bollworms in intact fruiting bodies as well as the boll and loculi damage in Bt sprayed plots as compared to other treatments.