Tannery waste is one of the most complex and putrescible organic industrial wastes causing huge amount of water pollution. Through the excessive organic load present in tannery wastes the oxygen content of the waters is depleted, which leads to the death of fish and other aquatic animals. In India, Tamil Nadu is one of the largest exporters of leather. Taking a cue of all this the impact of tannery effluent from Chingelpat district on various biochemical parameters viz. protein, carbohydrate and lipid content of the various tissues such as muscle, liver, ovary, skin, gill, brain, heart, intestine of larvivorous malaria control fish, Gambusia affinis has been studied.