An attempt has been made to study the soil bacteria, fungi and actinomycetes population in Seabuckthorn stand soil at different altitudinal zones in different seasons. Among different altitudinal zones greater microbial population size was recorded in Seabuckthorn stand soil of middle elevation zone followed by lower elevation zone and lower was found in upper elevation zone. Bacterial, fungal and actinomycetes population was comparatively more rhizospheric region of Seabuckthorm plant than non-rhizospheric soil. Non-Seabuckthorn site soil was always harboured less population of bacteria, fungi and actinomycetes compared to Seabuckthorn stand site soil.