Most religious beliefs involve some ceremonial use of 'holy' water and in India the water of the river Ganga is treated with such reverence. Scientists studied that the water of Ganga at its origin is in a pure state and even after being kept for several years, does not get contaminated. These medical properties of Ganga water are attributed to the medicinal secretions of herbs and mineral content which get mixed with the water. A study was conducted to validate our ancient knowledge about the antimicrobial effect of Ganga water and to evaluate the potential of Ganga water in our endeavor to explore the possibility of using Ganga water as a novel source of antimicrobial compounds.